Clinical Implication of ApoB (12669G/A) Gene Polymorphism and Risk of Cardio Vascular Disease in Indian Population

  • Farah Siddiqui Department of Biochemistry, Northern Border University, Arar, KSA
  • Sangeeta Singh Prof. & Head Department of Biochemistry, Govt. Medical College, Haldwani
  • Arun Joshi Prof. & Head Department of Cardiology, Govt. Medical College, Haldwani.
  • S R Saxena Prof. & Head Department of Medicine, Govt. Medical College, Haldwani
  • Ragini Srivastava Assistant Professor Department of Biochemistry, BHU IMS, Banaras U.P
  • Amit Jain Department of Biochemistry, Maulana Azad Medical College and Associated hospitals, New Delhi, India.
  • Prasant Yadav Department of Biochemistry, All India Institute of Medical Sciences, Jodhpur, India.
  • Mirza Masroor Department of Biochemistry, Maulana Azad Medical College and Associated hospitals, New Delhi, India.
Keywords: Cardio vascular disease, apoB polymorphism, Clinical importance.

Abstract

Background: Cardiovascular disease is rising day by day due to having high fat diet and due to genetic alterations. Materials & Methods: Study included 70 CVD patients and their peripheral blood samples were collected for genotyping by venipuncture under aseptic condition in EDTA vials (2ml) as well as in serum vials (3ml) for biochemical parameters. Genomic DNA extraction was done by phenol chloroform method from blood samples collected in EDTA vials from cases as well as controls for genotype study. Results: The difference of genotype between cases and controls was found to be significant (p=0.0003). Study observed that high percentage of GA 29 (41.4%) and AA 8 (11.4%) genotype was found in patients compared to controls GA 10 (20%) and AA 0 (0%) while lower GG 33 (47.2%) genotype in patients compared to control GG 40 (80%) genotype. Compared to the GG genotype, the OR 3.51 (1.49-8.25) and 20.55 (1.14-369.6) for the heterozygous GA and homozygous AA genotypes were estimated, suggesting a possible dominant effect of Apo B polymorphism on CVD risk. In smokers, compared to the GG genotype, the OR 2.19 (0.69-6.88) and 1.71 (0.29-9.87) for the heterozygous GA and homozygous AA genotypes. In alcoholism, compared to the GG genotype, the OR 2.66 (0.93-7.57) and 8.4 (0.92-76.19) for the heterozygous GA and homozygous AA genotypes. Patients with mutant  homozygous AA, heterozygous GA genotypes showed 123.3+14.34 (mg/dl) and 76.92+24.09 Apo B level in CVD patients compared to wild type GG homozygous genotypes were 70.82+17.12. Conclusion: It was observed that Apo B gene polymorphism and smoking behaviour found to be associated with increased risk of CVD in Indian population.

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Published
2018-03-21
How to Cite
1.
Siddiqui F, Singh S, Joshi A, Saxena S, Srivastava R, Jain A, Yadav P, Masroor M. Clinical Implication of ApoB (12669G/A) Gene Polymorphism and Risk of Cardio Vascular Disease in Indian Population. IABCR [Internet]. 21Mar.2018 [cited 11Dec.2018];4(1):202-5. Available from: https://iabcr.org/index.php/iabcr/article/view/310