Cypermethrin-induced Repeated-dose Immuno-toxicity in Oryctolagus Cuniculus: An Investigation into Pathology of Spleen and Blood

  • Latif Ahmad Baqai College of Veterinary Sciences, Baqai Medical University, Karachi-Pakistan,Department of Pathology, University of Agriculture, Faisalabad-Pakistan,Sargodha Medical College, University of Sargodha-Pakistan
  • Ahrar Khan Department of Pathology, University of Agriculture, Faisalabad-Pakistan
  • Muhammad Ismail Rind Baqai College of Veterinary Sciences, Baqai Medical University, Karachi-Pakistan
  • Wasim Ali Vistro Baqai College of Veterinary Sciences, Baqai Medical University, Karachi-Pakistan
  • Muhammad Asif Raza Baqai College of Veterinary Sciences, Baqai Medical University, Karachi-Pakistan
  • Tabinda Khawaja Livestock and Poultry Production, University of Poonch Rawalakot-Pakistan
  • Tahira Tabassum Sargodha Medical College, University of Sargodha-Pakistan
  • Imdad Hussain Department of Poultry Husbandry, Sindh Agriculture University, TandoJam-Pakistan
  • Khalid Hussain Baqai College of Veterinary Sciences, Baqai Medical University, Karachi-Pakistan
  • Sahib Shahani Ontario Network of Experts, Mississauga, Ontario-Canada
  • Muhammad Farooque Leghari Baqai College of Veterinary Sciences, Baqai Medical University, Karachi-Pakistan
  • Muhammad Tariq Javed Department of Pathology, University of Agriculture, Faisalabad-Pakistan
Keywords: Malpighian corpuscles, pesticide poisoning, lymphocyte production, macrophages, erythrocytes

Abstract

Background: Cypermethrin is a pyrethroid insecticide widely used in agriculture and medicines. Its toxicity effects on spleen and blood in male and female rabbits is studied in this research. Methods: Age- and weight-matched does/females (n=24) and bucks/males (n=24) of Japanese White rabbits were subjected to intraperitoneal cypermethrin administration @50, 100 and 150 mg.kg-1 b.wt. in groups B, C and D, respectively.  The experimental rabbit does and bucks were tested for hematology alterations after each of 5 CY-treatments, at days 1, 8, 15, 22 and 29.  One rabbit doe and one rabbit buck were sacrificed fortnightly (days 15, 29, 43, 57 and 71) to obtain spleen for histological studies. Results: CY-treated rabbit bucks developed anemia, leukopenia, neutropenia, monocytosis, eosinophilia and basocytopenia mostly in dose and time dependent manner.  In contrast, rabbit does show transient but significant leukocytosis, neutropenia, lymphopenia, monocytosis, eosinophilia and basophilia only in high dose group.  Moreover, spleen histology revealed congestion, depletion of white pulp with increased red pulp and hemosiderin deposition in CY treated rabbit bucks, but not rabbit does. Conclusions: This study concludes that immuno-toxicity by cypermethrin insecticide is not similar in male and female subjects.

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Published
2017-06-18
How to Cite
1.
Ahmad L, Khan A, Rind M, Vistro W, Raza M, Khawaja T, Tabassum T, Hussain I, Hussain K, Shahani S, Leghari M, Javed M. Cypermethrin-induced Repeated-dose Immuno-toxicity in Oryctolagus Cuniculus: An Investigation into Pathology of Spleen and Blood. IABCR [Internet]. 18Jun.2017 [cited 24Jul.2019];3(2):9-4. Available from: https://iabcr.org/index.php/iabcr/article/view/74