Haematological and Immunophenotypical Study on Acute Leukemias
Background: Acute leukemia comprises a heterogeneous group of diseases characterized by rapid and uncontrolled clonal expansion of progenitor cells of the hematopoietic system. Immunophenotyping of acute leukemia is one of the most important clinical applications of flowcytometry.
Methods: This study was conducted in Department of Pathology in , Chirayu Medical College and Hospital, Bhopal, Madhya Pradesh. This study was conducted over a period of one year. Total 104 cases were included in this study.
Results: In our study, 104 cases of diﬀerent patterns of acute leukemia were reported. There were total 62 cases of AML, 30 cases of B-cell ALL, 6 cases of T-cell ALL, 4 cases of bi-lineage and 2 case of bi-phenotypic leukemia.
Conclusion: It can conclude that immunophenotyping is an important diagnostic tool to diﬀerentiate between AML and ALL in morphologically challenging cases.
2. Ching Hon Pui. Childhood leukemias.48-51.
3. Neame PB, Soamboonsrup P, Browman GP et al. Classifying acute leukemia by immunophenotyping: a combined FAB-immunologic classification of AML. Blood. 1986; 68:1355-1362.
4. McCulloch EA. Stem cells in normal and leukemic hemopoiesis (Henry Stratton Lecture, 1982). Blood 1983;62:1-13.
5. Chana J. Shani T, Hashmi K. Role of immunophenotyping in diagnosis of acute leukemia. J Coll. Physician Surg. Pak 2000;15860.
6. Weir EG, Borowitz MJ. Flow cytometry in the diagnosis of acute leukemia. Seminars in hematology 2001;38:124 - 8.
7. Noor NA, Masood M. Clinico-epidemiological study of Leukemia in Multan: Pak J Med Research 1989; 28: 232-43.
8. Khalidi HS, Medeiros LJ, Chang KL, Brynes RK, Slovak ML, Arber DA.The immunophenotype of adult acute myeloid leukemia: High frequency of lymphoid antigen expression and comparison of immunophenotype, French-American-British classiﬁcation, and karyotypic abnormalities. Am J Clin Pathol 1998;109:211-20.
9. Ghosh S, Shinde SC, Kumaran GS, Sapre RS, Dhond SR, Badrinath Y, et al. Haematologic and immunophenotypic proﬁle of acute myeloid leukemia: An experience of Tata memorial hospital. Indian J Cancer 2003;40:71-6.
10. Shanta V, Maitreyan V, Sagar TG, Gajalakshmi CK, Rajalekshmy KR. Prognostic variables and survival in pediatric acute lymphoblastic leukemias: Cancer institute experience. Pediatr Hematol Oncol 1996;13:205-16.
11. Magrath I, Shanta V, Advani S, Adde M, Arya LS, Banavali S, et al. Treatment of acute lymphoblastic leukaemia in countries with limited resources; lessons from use of a single protocol in India over a twenty year period. Eur J Cancer 2005;41:1570-83.
12. Annual report. (1086-1987) Classiﬁcation and diagnosis of leukemias using cytochemical and immuuolgical maker technique. PMRC: 96-102.
13. Benc MC, Castoldi G, Knapp W et al. Proposal for the immunological classification of Acute Leukemias. Leukemia 1995;9:1783- 6.
14. Robins L, Kumar VD. e haematopoietic and lymphoid system. In: Basic Pathology. 4th Ed. Philadelphia: Saunders; 1987: p. 351-406.
15. Childs c.c; and Stass SA. Introduction, Characterization & Diagnosis of acute leukemia. In the Acute Leukemia. (ed by) Stass SA. New York and Basel. Marcel Dekker Inc; 1987: p.1-26
16. Bonnet D, John ED. Human AML is organized as a hierarchy that originates from a primitive haematopioetic cell. Nature Medicine Vol. 3 July 1997. p.730-37.
Copyright (c) 2021 Pooja Shrivastava
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.