Cypermethrin-induced Repeated-dose Immuno-toxicity in Oryctolagus Cuniculus: An Investigation into Pathology of Spleen and Blood


  • Latif Ahmad Baqai College of Veterinary Sciences, Baqai Medical University, Karachi-Pakistan,Department of Pathology, University of Agriculture, Faisalabad-Pakistan,Sargodha Medical College, University of Sargodha-Pakistan Author
  • Ahrar Khan Department of Pathology, University of Agriculture, Faisalabad-Pakistan Author
  • Muhammad Ismail Rind Baqai College of Veterinary Sciences, Baqai Medical University, Karachi-Pakistan Author
  • Wasim Ali Vistro Baqai College of Veterinary Sciences, Baqai Medical University, Karachi-Pakistan Author
  • Muhammad Asif Raza Baqai College of Veterinary Sciences, Baqai Medical University, Karachi-Pakistan Author
  • Tabinda Khawaja Livestock and Poultry Production, University of Poonch Rawalakot-Pakistan Author
  • Tahira Tabassum Sargodha Medical College, University of Sargodha-Pakistan Author
  • Imdad Hussain Department of Poultry Husbandry, Sindh Agriculture University, TandoJam-Pakistan Author
  • Khalid Hussain Baqai College of Veterinary Sciences, Baqai Medical University, Karachi-Pakistan Author
  • Sahib Shahani Ontario Network of Experts, Mississauga, Ontario-Canada Author
  • Muhammad Farooque Leghari Baqai College of Veterinary Sciences, Baqai Medical University, Karachi-Pakistan Author
  • Muhammad Tariq Javed Department of Pathology, University of Agriculture, Faisalabad-Pakistan Author



Malpighian corpuscles, pesticide poisoning, lymphocyte production, macrophages, erythrocytes


Background: Cypermethrin is a pyrethroid insecticide widely used in agriculture and medicines. Its toxicity effects on spleen and blood in male and female rabbits is studied in this research. Methods: Age- and weight-matched does/females (n=24) and bucks/males (n=24) of Japanese White rabbits were subjected to intraperitoneal cypermethrin administration @50, 100 and 150 b.wt. in groups B, C and D, respectively.  The experimental rabbit does and bucks were tested for hematology alterations after each of 5 CY-treatments, at days 1, 8, 15, 22 and 29.  One rabbit doe and one rabbit buck were sacrificed fortnightly (days 15, 29, 43, 57 and 71) to obtain spleen for histological studies. Results: CY-treated rabbit bucks developed anemia, leukopenia, neutropenia, monocytosis, eosinophilia and basocytopenia mostly in dose and time dependent manner.  In contrast, rabbit does show transient but significant leukocytosis, neutropenia, lymphopenia, monocytosis, eosinophilia and basophilia only in high dose group.  Moreover, spleen histology revealed congestion, depletion of white pulp with increased red pulp and hemosiderin deposition in CY treated rabbit bucks, but not rabbit does. Conclusions: This study concludes that immuno-toxicity by cypermethrin insecticide is not similar in male and female subjects.


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Author Biography

  • Muhammad Ismail Rind, Baqai College of Veterinary Sciences, Baqai Medical University, Karachi-Pakistan



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