A Comparative Evaluation of Rapid Card Test with Enzyme- Linked Immunosorbent Assay for the Detection of HBsAg Among Pregnant Women in a Tertiary Care Hospital

  • Megha Sharma Assistant Professor, Dept. of Microbiology, Ananta Institute of Medical Sciences Research Centre, Rajsamand
  • Saroj Golia Professor, Dept. of Microbiology, Ananta Institute of Medical Sciences Research Centre, Rajsamand
  • S K Mehra Ex- Professor & Head, Department of Microbiology, Geetanjali Medical College & Hospital, Udaipur
  • Manoj V Jani Assistant Professor, Department of TB & Chest, Ananta Institute of Medical Sciences Research Centre, Rajsamand
Keywords: HBsAg, Immunochromatographic kit ICT, ELISA, comparison for effectiveness.

Abstract

Background: Hepatitis B virus infection is an important health problem and a leading cause of death worldwide. The most important marker for HBV infection is HBsAg. A proper identification of kit is required for proper diagnosis of infection, as well as disease management and prevention. Aims& Objective: - The aim of the present study was to compare rapid HBsAg detection ICT kits for the screening of HBV infection with gold standard ELISA method.

Methods: The study comprised 500 blood samples of pregnant women. These samples were tested with ICT kits of (Hepa Card Company) and ELISA (HEPLISA by J. Mitra.). Results: In present study we found 100% sensitivity, 99.59% specificity, 81.81% positive predictive value ,100%Negative predictive value and 99.60% diagnostic efficiency of rapid card test with comparison to ELISA for HBsAg detection. Conclusion: The present study concluded that the overall performance of the rapid ICT kit for HBsAg was equally sensitive to ELISA and yet they were cheap and quicker. It can be recommended that ELISA comparable rapid devices may be allowed to be used for preliminary screening of HBsAg especially in remote areas or where cost is an issue.

Downloads

Download data is not yet available.

References

1. Dwivedi M, Misra SP, Misra V. Seroprevalence of hepatitis B infection during pregnancy and risk of perinatal transmission. Indian J Gastroenterol 2011; 30:66-71.
2. Okada K, Yamada T, Miyakawa Y, Mayumi M. Hepatitis B surface antigen in the serum of infants after deliver from asymptomatic carrier mothers. J Pediatr 1975; 87:360-3.
3. Tosun S Y, Yuceturk M, Benzergil S. The immunization of babies born of HBsAg positive pregnant women. Ege Tip Dergisi. 2002;41(1):21–23.
4. Uyar Y, Cabar C, Balci A. Seroprevalence of hepatitis B virus among pregnant women in Northern Turkey. Hepatitis Monthly. 2009;9(2):146–149.
5. Arfaoui D, Fkih M, Hafsa AE, Kaabia N, Azzouz M. Hepatitis B and pregnancy. Tunis Med. 2010;88(6):383–389. [PubMed]
6. Khan S, Attaullah S.share of Afghanistan populace in hepatitis b and hepatitis c infection’s pool: is it worthwhile. Virol J 2011;8:216-22.
7. Nanu A, Sharma SP, ChatterJee K, et al. Markers for Transfusion Transmissible diseases in Northern Indian Voluntary and Replacement Blood Donors: Prevalence and Trends. Vox Sang. 1997; 73: 70–3p.
8. World Health Organization. Status of blood safety in the WHO African Region. Report of the 2004 Survey WHO Regional Office for Africa, Brazzaville. Geneva: WHO; 2007.
9. Torane VP, Shastri JS. Comparison of ELISA HBSAg and HCV among healthy blood donors in a tertiary Care Hospital in Mumbai. Indian J Med. Microbiol. 2008;2: 284–5p.
10. Rahman M, Khan SA, Lodhi Y. Unconfirmed reactive screening tests and their impact on donor management. Pak J Med Sci. 2008; 24: 517–19p.
11. Allain JP, Lee H. Rapid tests for detection of viral markers in blood transfusion. Expert Rev Mol Diagn. 2005; 5: 31–41p.
12. Raj AA, Subramaniam T, Raghuraman S, et al. Evaluation of an indigenously manufactured rapid immunochromatographic test for detection of HBsAg. Ind J Pathol Microbiol. 2001; 44: 413–14p.
13. Yogendra K. Tiwari, Smariti Pundir, Gaurav Saraf, Kavita Pawan, Deepti Dashora, Monish Pokra, Vasudev Patidar, Jyoti Rana, R.K. Mishra*, A Comparison of Rapid Card Test with Enzyme-Linked Immunosorbent Assay for the Detection of Hepatitis B Surface Antigen [HBsAg] in Tertiary Care Hospital, RRJoMV (2017) 27-31 © STM Journals 2017. All Rights Reserved
14. Kaur H, Dhanao J, Oberoi A. Evaluation of rapid kits for detection of HIV, HBsAg and HCV infections. Ind J Med Sci. 2000; 54: 432–4p.
15. Zahoorullah, Akhtar T, Najib ul Haq, et al. Latex agglutination and immuno- chromatographic screening tests verses reverse passive hem-agglutination for B surface antigen in serum. Pakistan Journal of Medical Research. 2001; 40: 69–71p.
16. Ansari MHK, Omrani MD, Movahedi V. Comparative evaluation of immuno- chromatographic rapid diagnostic tests and PCR methods for detection of human hepatitis B surface antigen. Hepatitis Monthly. 2007; 7: 87–91p.
17. Lin Y, Wang Y, Loua A, et al. Evaluation of a new hepatitis B virus surface antigen rapid test with improved sensitivity. J Clin Microbiol. 2008; 46: 3319– 24p.
18. Qasmi SA, Aqeel S, Ahmed M, et al. Detection of Hepatitis B Viruses in Normal Individuals of Karachi. J Coll Physicians Surg Pak. 2000; 10: 467–9p. 19. Irwig L, Bossuyt P, Glasziou P, et al. Designing studies to ensure that
estimates of test accuracy are transferable. BMJ. 2002; 324: 669–71p.
20. Kane M. Global Plan of Action for Hepatitis B Immunization: Global Program for Vaccine and Immunization. Expanded Program on Immunization. Geneva:
World Health Organization, 1994.
21. Gul N, Sarwar J, Idris Muhammad, et al. Seroprevalence of hepatitis C in
pregnant females of hazara division. Journal Ayub Medical College
Abbottabad. 2009; 21: 83–6p.
22. Ansari MHK, Omrani M, Movahedi V. Comparative Evaluation
Immunochromatographic rapid Diagnostic tests (Strip and Device) and PCR methods for Detection of Human Hepatitis B surface Antigens. Hepat Mon 2007;7(2):87-91.
23. Maity S, Nandi S, Biswas S,et al. Performance and diagnostic usefulness of commercially available enzyme linked immunosorbent assay and rapid kits for detection of HIV, HBV and HCV in India. Virol J 2012; 9:290-8.
24. Chameera EWS, Noordeen F, Pandithasundara H, et al. Diagnostic efficacy of rapid assays used for the detection of Hepatitis B virus surface antigen. Sri Lankan Journal of Infectious Diseases 2013; 3(2):21-7.
25. Scheiblauer H, El-Nageh M, Diaz S,et al.Performance evaluation of 70 Hepatitis B virus(HBV) surface antigen (HBsAg) assays from around the world by a geographically diverse panel with an array of HBV genotypes and HbsAg subtypes. Vox Sang 2010; 98:403-14.
26. Torane VP, Shastri JS. Comparision of ELISA and Rapid screening test for the diagnosis of HIV, HBV and HCV among healthy blood donor in a tertiary care hospital in Mumbai. Ind J Med Microbiol. 2008; 2: 284–5p.
Published
2019-03-21
How to Cite
1.
Sharma M, Golia S, Mehra S, Jani M. A Comparative Evaluation of Rapid Card Test with Enzyme- Linked Immunosorbent Assay for the Detection of HBsAg Among Pregnant Women in a Tertiary Care Hospital. IABCR [Internet]. 21Mar.2019 [cited 20Jun.2019];5(1):31-3. Available from: https://iabcr.org/index.php/iabcr/article/view/461