A Study of Aerobic Bacterial Isolates and their Antibiotic Susceptibility Pattern from Pus Samples in a Tertiary Care Hospital, Rajasthan

  • Ritu Bhatnagar Assistant Professor, Pacific Medical College and Hospital, Udaipur, Rajasthan
  • Pragnesh Patel Assistant Professor, Pacific Medical College and Hospital, Udaipur, Rajasthan
Keywords: Aerobic bacteria, pus isolates, pus culture and sensitivity

Abstract

Background: Aerobic bacteria are the major cause of pyogenic infections characterized by local and systemic inflammation and pus formation. Different studies show that bacterial profile of pus samples and their antimicrobial sensitivity pattern shows many variations. Emergence of antimicrobial drug resistance has made the treatment of pyogenic infections difficult. Pus culture and sensitivity testing done prior to start antibiotic therapy help in right approach towards selection of antibiotic and treatment.

Methods: A study was conducted in Department of Microbiology of a private tertiary care hospital in Udaipur, Rajasthan from August 2017 to January 2018. Total of 240 pus samples were analyzed for aerobic culture and sensitivity. Processing and identification was done as per standard guidelines. Antibiotic sensitivity testing was performed by Kirby Baur disc diffusion method.

Results: In our study, out of 240 pus samples sent from various departments, 160 (66.6%) were positive for bacterial growth. Commonest isolate was Staphylococcus aureus, followed by Pseudomonas species, E. coli, Klebsiella species, Enterococcus species, either alone or in mixed growth. All Staphylococcus aureus were sensitive to Vancomycin and Linezolid. For Pseudomonas species, effective drugs were piperacillin-tazobactem and polymyxin B; for enterobacteriacae, most effective drug was meropenem.

Conclusion: It is of utmost importance for a clinician to send the pus sample for microbiological analysis and antibiotic sensitivity testing before starting antibiotic therapy to minimize the emergence of drug resistance.

Downloads

Download data is not yet available.

References

1. L. Cogen, V. Nizet, R. L. Gallo. Skin microbiota: a source of disease or defence?. Br J Dermatol 2008; 158(3): 442-455.
2. Dryden MS. Complicated skin and soft tissue infection. J Antimicrob Chemother November 2010; 65(3): 35-44.
3. A Biradar, F Farooqui, R Prakash, S Y Khaqri, I Itagi. Aerobic bacteriological profile with antibiogram of pus isolates. Indian J Microbiol Res 2016; 3(3): 245-249.
4. M. Chauhan, Manish S, S. Mahajan. Aerobic Bacterial Profile and antibiotic sensitivity pattern of pus isolates in a tertiary care Hospital. Int.J.Curr.Microbiol.App.Sci 2015; 4(5): 784-787.
5. Collee JG, Miles RS, Watt B. Tests for identification of bacteria In: Mackie and Mc Cartney’s Practical Medical Microbiology (Collee JG, Fraser AG, Marmion BP, Simmons A, eds.), 14 edition, London: Churchill Livingstone, 1996; 131-45.
6. Clinical and laboratory standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing: 21st Informational supplement 2011. CLSI document M100 –S20 wayne. PA: CLSI: 2011.
7. Iredell J, Brown J, Tagg K. Antibiotic resistance in Enterobacteriaceae: mechanisms and clinical implications. British Medical Journal 2016; 352: h6420.
8. Roopa C, Deepali V. PUS culture isolates and their antibiotic sensitivity at a Tertiary Care Hospital in Hyderabad Karnataka Region. International Journal of Medical Microbiology and Tropical Diseases 2017; 3(4): 140-145.
9. Mama M, Abdissa A, Sewunet T. Antimicrobial susceptibility pattern of bacterial isolates from wound infection and their sensitivity to alternative topical agents at Jimma University Specialized Hospital, South-West Ethiopia. Ann Clin Microbiol Antimicrob. 2014; 13: 14.
10. Agnihotri N, Gupta V, Joshi RM. Aerobic bacterial isolates from burn wound infections and their antibiograms--a five-year study. Burns 2004; 30(3): 241-3.
11. Basu S, Ramchuran Panray T, Bali Singh T, Gulati AK, Shukla VK. A prospective, descriptive study to identify the microbiological profile of chronic wounds in outpatients. Ostomy Wound Manage 2009; 55(1): 14-20.
12. G. Suguneswari, A. Heraman Singh, Ranjan Basu. Bacteriological profile of osteomyelitis in a tertiary care hospital at Visakhapatnam, Andhra Pradesh. IJCRR 2013; 5(20): 52-58.
Published
2018-09-30
How to Cite
1.
Bhatnagar R, Patel P. A Study of Aerobic Bacterial Isolates and their Antibiotic Susceptibility Pattern from Pus Samples in a Tertiary Care Hospital, Rajasthan. IABCR [Internet]. 30Sep.2018 [cited 11Dec.2018];4(3):19-1. Available from: https://iabcr.org/index.php/iabcr/article/view/395