Is the NMDA Receptor of the CA1 Region Participated in the Amnesic Effect of Harmane?

  • Mohammad Nasehi Medical Genomics Research Center and School of Advanced Sciences in Medicine, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran
  • Sara Akhlagi Department of Biology, Faculty of Basic Sciences, Northern branch, Islamic Azad University, Tehran, Iran
  • Fatemeh Khakpai Institute for Cognitive Science Studies (ICSS), Tehran, Iran, 4Department of Pharmacology School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Mohammad Reza Zarrindast Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Keywords: NMDA, D-AP7, Harmane, CA1, Memory


Aims: In the present study, we investigated the influence of NMDA receptor agonist (N-methyl-d-aspartate) and antagonist (D-AP7) on amnesia induced by a β-carboline alkaloid, harmane.

Methodology: Animals implanted with bilateral cannulae at the CA1 regions of the dorsal hippocampus and microinjected with glutamatergic drugs. One-trial step-down was used to assess memory acquisition and then, the hole-board method to assess exploratory behaviors in adult male NMRI mice. Results: The results revealed that pre-training intra-CA1 administration of NMDA (0.5 ng/mouse) and D-AP7 (0.25 and 0.5 ng/mouse) improved and impaired memory acquisition, respectively. Also, pre-training intra-peritoneal (i.p.) administration of harmane (12 mg/kg) decreased memory acquisition. Furthermore, pre-training intra-CA1 injection of sub-threshold dose of NMDA (0.02 ng/mouse) reversed, while non-significant dose of D-AP7 (0.125 ng/mouse) did not change impairment of memory acquisition induced by harmane (12 mg/kg, i.p.). Conclusion: In addition, all above doses of drugs did not alter locomotor activity. These results suggest that the CA1 NMDA receptors are involved in harmane-induced amnesia. 


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How to Cite
Nasehi M, Akhlagi S, Khakpai F, Zarrindast MR. Is the NMDA Receptor of the CA1 Region Participated in the Amnesic Effect of Harmane?. Int Arch BioMed Clin Res [Internet]. 2018Feb.15 [cited 2020Aug.12];2(2):53-8. Available from: