Comparison of Immunogenic Effect of Three Oil Adjuvant Vaccines against Haemorrhagic Septicaemia in Cattle and Buffalo

  • Hina Afroz Veterinary Research Institute, Zarrar Shaheed Road, Lahore, Pakistan.
  • Sumiyya Sattar Veterinary Research Institute, Zarrar Shaheed Road, Lahore, Pakistan.
  • Bushra Zamir Veterinary Research Institute, Zarrar Shaheed Road, Lahore, Pakistan.
  • Ikram ul Haq Institute of Industrial Biotechnology, Government College University, Lahore, Pakistan.
  • Rehan Rafique Veterinary Research Institute, Zarrar Shaheed Road, Lahore, Pakistan.
  • Asfa Rasool Veterinary Research Institute, Zarrar Shaheed Road, Lahore, Pakistan.
Keywords: Haemorrhagic Septicaemia, IHA, cattle, buffalo, montanide, ISA 206, ISA-50, liquid paraffin, lanolin.GMT

Abstract

Haemorrhagic Septicaemia is one of the most common, fatal and acute bacterial diseases of livestock which causes mortality above 70% and is caused by Pasteurella multocida. The only satisfactory and practical method of control and prevention is timely vaccination of all the healthy and in contact animals. Different types of vaccines are being used for the immunity against this disease. In this project three oil based vaccines were produced. Two single emulsion vaccines were prepared by utilizing Montanide ISA-50 and liquid paraffin with lanolin where as one double emulsion with the help of Montanide ISA-206 was prepared. In house quality control testing and safety testing was performed on swiss albino mice. For immune titre IHA was performed by collecting serum from each and every animal including control animals. The comparison of IHA was done via statistical analysis by using GMT, Single emulsion vaccine prepared from liquid paraffin with lanolin gave maximum immune titre out of all the three vaccines in large animals and in young calves ISA 206 gave a significant titre.

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References

1. Sawada T, Rimler R B, Rhoades K P. Haemorrhagic septicaemia, naturally acquired antibodies against Pasteurella multocida types B and E in calves in the United States. Am J Vet Res 1985; 46: 1247–1250.
2. Anonymous. OIE manual of diagnostic tests and vaccines for terrestrial animals, office international des epizooties 2008; 1: 739-751.
3. Sheikh MA, Azam M, Shakoori AR. Observations on haemorrhagic septicaemia in Pakistan livestock. Zentralbl Veterinarmed Bulletin 1996; 43: 293-304.
4. De Alwis MCL. Haemorrhagic septicaemia. A general review.Br vet J 1992; 148: 99-112.
5. Boyce JD, Chung JY & Adler B. Genetic organization of the capsule biosynthetic locus of Pastuerella multocida M1404 (B:2). Vet Microbiol. 2000; 72: 121-134.
6. Umer F, Zaka S, Khan MA, Ali I and Qamar MF. Sero-surveillance of hemorrhagic septicemia in buffaloes and cattle in Southern Punjab, Pakistan. Pak Vet J 2011; 31(3): 254-256.
7. Tasneem K. Studies on h.s oil adjuvant vaccine prepared by dense culture of Pasteurella multocida Robert type-I on improved culture medium. MSc.(Hons.), University of Agriculture, Faisalabad, Pakistan, 1993.
8. Aucouturier J, Dupuis L, Ganne V. Adjuvant designed for veterinary and human vaccines. Vaccine 2001; 19: 2666-2672.
9. Reddy GS, Srinivasan VA. Potency of haemorrhagic septicaemia vaccine. Indian j of Ani Sci 1995; 65(10): 1100-1101.
10. Nawaz S. Preparation and Evaluation of a Pastuerella multocida vaccine. MSc.(Hons.), University of Agriculture, Faisalabad, Pakistan, 1993.
11. Kayani RFI, Naqvi ZH, Chaudhry TM, Shauket M. Studies on Pasteurella multocida: Indirect haemagglutination test for the identification of serological types. Pakistan J Bio Sci 2000; 3(3): 503-504.
12. Shah AH, Kamboh AA, Rajput N, Korejo NA. Optimization of physico-chemical conditions for the growth of Pasteurella multocida under in Vitro. J Agri Soc Sci 2008; 4(4): 176-179.
13. Sarwar N, Muhammad K, Rabbani M, Younus M, Sarwar M, Ali MA, Hanif K, Kamran M. Optimization of physico-chemical factors augmenting in vitro biomass production of Pasteurella multocida. The J of Animal & Plant Sci 2013; 23(4): 1085-1088.
14. Jaffri KT, Gill ZJ, Bhatti AR, Raza A. Immune response of buffalo calves to haemorrhagic septicemia oil adjuvant and alum precipitated vaccine. Inter J of Agri and Bio 2006; 8(5): 645–647.
15. Jabbari AR, Moazeni Jula GR. Improvement of haemorrhagic septicaemia vaccine by removing of anaphylactic agents. Arch Razi Ins 2002; 54: 85-92.
16. Namioka S, Murata M. Serological studies on Pasteurella multocida IV. Characteristics of the somatic “O” antigen. Cornell Vet 1964; 51:522.
17. Fraser J, Donachie W, Quire M, Gilmour NJL. Rapid indirect haemagglutination test for serotyping Pasteurella haemolytica. J Clin Microbiol 1983; 118: 206–207.
18. Mohi-ud-din, Mudasser H, Zahid I, Iftikhar H. Immune response of rabbits to hemorrhagic septicemia vaccine formulations adjuvanted with montanide ISA-206, paraffin oil and alum. Asian J Agri Biol 2014; 2(2): 161-167.
Published
2016-06-18
How to Cite
1.
Afroz H, Sattar S, Zamir B, Haq I ul, Rafique R, Rasool A. Comparison of Immunogenic Effect of Three Oil Adjuvant Vaccines against Haemorrhagic Septicaemia in Cattle and Buffalo. Int Arch BioMed Clin Res [Internet]. 2016Jun.18 [cited 2019Dec.14];2(2):43-8. Available from: https://iabcr.org/index.php/iabcr/article/view/209
Section
ORIGINAL ARTICLES ~ General Surgery