A Study of Genotypic Characterisation (mec-A Gene & Panton Valentine Leukocidin Gene) of Methicillin Resistance Staphylococcus aureus Strains Isolated From Tertiary Care Hospital
Background: MRSA causes globally severe morbidity and mortality. Methicillin resistance is caused by the presence of mecA gene, which has a low affinity for β-lactam antibiotics, whereas absence of the gene from Staphylococcal strain indicates methicillin susceptibility. Cefoxitin is a potent inducer of the mecA regulatory system. The PVL gene has been detected in skin associated hospital acquired infection.
Aims and Objectives: To isolate methicillin resistant Staphylococcus aureus from various clinical specimens from hospital acquired infections and study the association of mecA and PVL gene in MRSA from tertiary care hospital.
Materials and Methods: Processing of specimens were done according to standard bacteriological procedures and identification of bacterial isolate was done by colony morphology & biochemical test and further subjected to antibiotic susceptibility testing and to Polymerase chain reaction for detection of mec A gene and PVL gene.
Results: Testing with cefoxitin disk diffusion method identified 162 isolates as MRSA, by PCR, mec-A gene was present in 162 isolates and 32 isolates of MRSA showed PVL gene and both the genes were present in 21 isolates of MRSA.
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