Evaluation of Some Mushrooms from Kashmir Valley for their Potential Anti-Cancer Activity
DOI:
https://doi.org/10.21276/tv6wa319Keywords:
Evaluation, mushrooms, anti- cancer, extract, screeningAbstract
Background: In the recent past, there has been a shift towards alternate and traditional therapies for the prevention and cure for various diseases including cancer, hypertension, diabetes etc. Due to the risk of side effects associated with allopathic medicines, people are fast turning towards traditional and folk medicines because of their supposedly low risk of side effects. Mushrooms have a very long and well-established role in traditional system of medicine in many Asian countries especially in China, Korea, and Japan etc.
Methods: Fresh mushrooms were collected and identified based on morphological and reproductive characters by comparing with standard field guides by Largent (1973).Three different mushroom species were selected for the study. The material collected from target sites were subjected to solvent extraction followed by preparation of stock extracts, which were further used for the evaluation of anti-cancer activity. In-vitro cytotoxicity against different human cell lines was determined and the samples showing 50% or more growth inhibition at 100 µg/ml were considered as potential.
Results: Ethanolic extract of mushroom 1 (100µg/ml) exhibited highest % age growth inhibition of 61, 53, 25% on lung, colon and CNS cell lines respectively. In case of mushroom-2 Ethanol: water (1:1) extracts 1 (100 µg/ml) showed highest %age growth inhibition of 19, 38, 7% against liver, Neuroblastoma, Colon cell lines respectively. Likewise, Ethanolic Extract of mushroom-3(100 µg/ml) exhibited highest %age growth inhibition of 58, 65, 13% on lung, neuroblastoma and prostate cell lines respectively.
Conclusions: Based on preliminary evaluation two of the mushroom varieties showed promising results in terms of their anticancer activity however they need further evaluation and determination to ascertain their potential anticancer activity.
Downloads
References
Wasser SP. Medicinal mushrooms as a source of antitumor and immunomodulatory polysaccharides. Appl. Microbiol. Biotechnol., 2002, 60, 258-274.
Ferreira I, A Vaz J, Vasconcelos MH, Martins A. Compounds from wild mushrooms with antitumor potential. Anti-Cancer Agents in Medicinal. 2010 Jun 1; 10(5):424-36.
Nidhi A, and Chowdhry PN. Anti-tumour potential of active compounds (polysaccharides) of wild mushrooms from Rajouri dist. of Jammu & Kashmir, India. IJPSR, 2013, 4, 11-15.
Hawksworth DL. Mushrooms: the extent of the unexplored potential. J. Med. Mushrooms, 2001; 3, 333-337.
Poucheret P, Fons, F, Rapior S. Biological, and pharmacological activity of higher fungi: 20-year retrospective analysis. Mycologie, 2006, 27, 311-333.
Ajith TA, Janardhanan KK. Indian medicinal mushrooms as a source of antioxidant and antitumor agents. J. Clin. Biochem. Nutr., 2007,40, 157-162.
Smith AH, Weber NS. The mushroom hunter's field guide. University of Michigan Press; 1980.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of immunological methods. 1983 Dec 16;65:55-63.
Mizuno T. Bioactive biomolecules of mushrooms. Food function and medicinal effect of mushroom fungi. Food Rev. Int., 1995, 11,7-21.
Zaidman B, Yassin M, Mahajana J, Wasser SP. Medicinal mushroom modulators of molecular targets as cancer therapeutics. Appl. Microbiol. Biotechnol., 2005,67, 453-468
Bobek P, Galbavý Š. Hypocholesterolemic and antiatherogenic effect of oyster mushroom (Pleurotus ostreatus) in rabbits. Food/Nahrung. 1999 Oct 1;43(5):339-42.
Bobek P, Galbavy S. Effect of pleuran (beta-glucan from Pleurotus ostreatus) on the antioxidant status of the organism and on dimethyl hydrazine-induced precancerous lesions in rat colon. Br. Biomed. Sci., 2001,58, 164-168.
Manzi P, Aguzzi A, Pizzoferrato L. Nutritional value of mushrooms widely consumed in Italy. Food chemistry. 2001 May 31;73(3):321-5.
Brzin J, Rogelj B, Popovic T, Strukelj B, Ritonja A. Clitocypin, a new type of cysteine proteinase inhibitor from fruit bodies of mushroom Clitocybe nebularis. J. Biol. Chem., 2000, 275, 20104-20109.
Galesa K, Thomas RM, Kidrica M, Pain RH. Clitocypin, a new cysteine proteinase inhibitor, is monomeric: impact on the mechanism of folding. Biochem. Biophys. Res. Commun., 2004,324, 576-578.
Hobbs C Medicinal mushrooms: an exploration of tradition, healing and culture. Botanica Press, Santa Cruz, Calif (1995)
Stamets P Growing gourmet and medicinal mushrooms, 3rd edn.(2000) Ten Speed Press, Berkeley, Calif.
Wang G, Zhang J, Mizuno T, Zhuang C, Ito H, Mayuzumi H,Okamoto H, Li J (1993) Antitumor active polysaccharides from the Chinese mushroom Songshan Lingzhi, the fruiting body of Ganoderma tsugae. Biosci Biotechnol Biochem 57:894–900
Downloads
Published
Issue
Section
License
Copyright (c) 2024 International Archives of BioMedical and Clinical Research

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors are required to sign and submit the completed “Copyright transfer Form” upon acceptance of publication of the paper. This is determined by a publishing agreement between the author and International Archives of Biomedical and Clinical Research. These rights might include the right to publish, communicate and distribute online. Author(s) retain the copyright of their work. International Archives of Biomedical and Clinical Research supports the need for authors to share, disseminate and maximize the impact of their research.