Immunoexpression and Mutation Status of BRAF V600E in Papillary Thyroid Carcinoma
DOI:
https://doi.org/10.21276/p3h86w11Keywords:
Myanmar, BRAF V600E mutation, papillary thyroid carcinoma, formalin-fixed paraffin-embedded tissue, immunohistochemistry (IHC), amplification refractory mutation system- polymerase chain reaction, ARMS-PCRAbstract
Background: BRAF V600E mutation, with incidence rates ranging from 29% to 83% of papillary thyroid carcinoma (PTC), is the most common genetic mutation. Several methods have been used to detect this mutation but the determination of the rapid and easily available techniques is still needed. Thus, the aim was to describe the diagnostic performance of faster BRAFV600E-specific immunohistochemical method (IHC), compared to the amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) technique.
Methods: The histologically proven, paraffin-embedded tissue blocks of 44 papillary thyroid carcinoma patients who attended the Otorhinolaryngology, Head and Neck Surgery Specialist Hospital, Yangon, were collected. Anti-BRAF V600E monoclonal antibody was used for the IHC of tissue sections. For ARMS-PCR, the BRAF V600E mutation was detected after DNA extraction, followed by genomic DNA amplification using the QIAGEN Taq PCR Core kit.
Results: The mutation was found in 77.3% (34/44) and 75% (33/44) cases using IHC and ARMS-PCR, respectively. A total of 98% concordance was found between IHC and ARMS-PCR (43/44 tests). IHC results showed 100% (33/33) sensitivity, 91% (10/11) specificity, 97% (33/44) positive predictive values), and 100% (10/10) negative predictive values.
Conclusion: The results demonstrated that BRAF V600E IHC possesses the appropriate sensitivity and specificity for diagnostic use. This method could be considered a reliable preliminary screening technique in addition to a molecular approach for BRAF V600E mutation detection in papillary thyroid carcinoma patients on a routine basis.
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